Purification and characterization of the lethal toxin (alpha-toxin) of Clostridium septicum
نویسندگان
چکیده
منابع مشابه
Alpha Toxin Purification and Antibody Production Against Local Strain of Clostridium septicum NH2
BACKGROUND: Clostridium septicum has played a significant role as a causative agent of many acute fetal diseases in man and animals. Alpha- toxin is the main factor in the pathogenesis of C. septicum with hemolytic, necrotic and lethal activities. OBJECTIVES: The study was designed to evaluate alpha-toxin purification and antibody production rate against a local strain of C. septicum NH2 which ...
متن کاملMolecular cloning of Clostridium septicum vaccine strain alpha toxin gene in E. coli
Clostridium septicum a Gram positive anaerobic bacterium produces several toxins including alpha, beta, gamma and delta. C. septicum alpha toxin is lethal and is responsible for a serious disease known as gas gangrene. The aim of the present study was to molecular cloning and sequencing of C. septicum vaccine strain alpha toxin gene. Genomic DNA was extracted using standard phenol and chlorofor...
متن کاملThe Partial Purification of the Lethal Toxin of Clostridium Histolyticum *
Although several bacterial toxins have been prepared in highly purified form (c.f. Pappenheimer,6 Lamanna et al,,' and Pillemer8), comparatively little effort has been made in this direction with the toxins of the gas gangrene organism C. histolyticurm. Weinberg and Seguinll demonstrated that it could be precipitated with ammonium sulfate, and made the observation that ethanol destroys the toxi...
متن کاملClostridium septicum alpha-toxin is proteolytically activated by furin.
Clostridium septicum alpha-toxin is secreted as an inactive 46,450-Da protoxin. The protoxin is activated by proteolytic cleavage near the C terminus, which eventually causes the release of a 45-amino-acid fragment. Proteoytic activation and loss of the propeptide allow alpha-toxin to oligomerize and form pores on the plasma membrane, which results in colloidal-osmotic lysis. Activation may be ...
متن کاملClostridium septicum alpha toxin uses glycosylphosphatidylinositol-anchored protein receptors.
The alpha toxin produced by Clostridium septicum is a channel-forming protein that is an important contributor to the virulence of the organism. Chinese hamster ovary (CHO) cells are sensitive to low concentrations of the toxin, indicating that they contain toxin receptors. Using retroviral mutagenesis, a mutant CHO line (BAG15) was generated that is resistant to alpha toxin. FACS analysis show...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1992
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.60.3.784-790.1992